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<div id="7nbvr5v" class="tag"> <h1 class="content-title content-title-3 cmseasyedit text-center" cmseasy-id="857" > 凍干機：微生物菌種冷凍干燥前準備</h1> <style type="text/css"> h1.content-title-3 { line-height:1.6; display:block !important; font-size:22px; color:#333333; } h1 .content-title-3:hover { color:#333333; } </style> </div> <div id="jhrprl3" class="clearfix"> </div> <div id="dbfjplr" class="tag" style="margin-top: 15px; margin-bottom: 15px;"> <div id="vtlh7jl" class="visual-level clearfix visual-level-3"> </div> <style type="text/css"> .visual-level-3 { height: 1px; background:#eeeeee; } .visual-level-3:hover { background:#eeeeee; } </style></div> <div id="vph7jfl" class="clearfix"> </div> <div id="vddrn13" class="tag"> <p class="content-subtitle content-subtitle-3 cmseasyedit text-center" cmseasy-id="857" > </p> <style type="text/css"> .content-subtitle-3 { display:block !important; font-size:14px; color:#333333; } .content-subtitle-3:hover { color:#333333; } </style> </div> <div id="vb7fxpv" class="clearfix"> </div> <div id="jd7bpvl" class="tag"> <link href="/template_admin/admin/visual/sections/common/print/css/style.css" rel="stylesheet"> <div id="97vdzrn" class="claerfix col-md-12"> <p class="visual-print-tools visual-print-tools-3 text-right"> <a href="javascript:CallPrint('print');"> <i class="glyphicon glyphicon-print"></i> </a> <a href="javascript:doZoom(14)"> <span id="z7fhftp" class="column-title cmseasyedit" > 小 </span> </a> <a href="javascript:doZoom(18)"> <span id="xntvnhl" class="column-title cmseasyedit" > 中 </span> </a> <a href="javascript:doZoom(20)"> <span id="vrzfjjz" class="column-title cmseasyedit" > 大 </span> </a> </p> </div> <style type="text/css"> .visual-print-tools-3 { background:rgba(255, 255, 255, 0); } .visual-print-tools-3:hover { background:rgba(255, 255, 255, 0); } .visual-print-tools-3 a { font-size:14px; color:#333333; border-color:rgba(255, 255, 255, 0); border-radius: 0px; background:rgba(255, 255, 255, 0); } .visual-print-tools-3 a:hover { color:#000000; border-color:rgba(255, 255, 255, 0); border-radius: $_link-border-hover-radius; background:rgba(255, 255, 255, 0); } </style> <script src="/common/plugins/font-print/c_tool.js"></script></div> <div id="bx55dff" class="clearfix"> </div> <div id="735l3jv" class="tag"> <link href="/template_admin/admin/visual/sections/content/content/css/style.css" rel="stylesheet"> <div class="lrvt7hn" id="print" class="claerfix content-text content-text-3"> <span id="fnx7bnp" class="cmseasyedit content" cmseasy-id="857" > <p> 隨著<a target="_self">凍干機</a>技術不斷發(fā)展，通過凍干機將微生物菌種冷凍干燥保藏已證明是比較有效可行的方法。微生物菌種冷凍干燥前準備，主要有如下三個：</p><p>1、安瓿管準備</p><p> 安瓿管材料以中性玻璃為宜。清洗安瓿管時，先用2%鹽酸浸泡過夜，自來水沖洗干凈后，用蒸餾水浸泡至pH中性，干燥后、貼上標簽，標上菌號及時間，加入脫脂棉塞后，121℃下高壓滅菌15-20分鐘，備用。</p><p>2、保護劑的選擇和準備</p><p> 保護劑種類要根據微生物類別選擇。配制保護劑時，應注意其濃度及pH值，以及滅菌方法。如血清，可用過濾滅菌；牛奶要先脫脂，用離心方法去除上層油脂，一般在100℃間歇煮沸2-3次，每次10-30分鐘，備用。</p><p>3、凍干樣品的準備</p><p> 在最適宜的培養(yǎng)條件下將細胞培養(yǎng)至靜止期或成熟期，進行純度檢查后（參見科技部自然科技資源平臺聯合管理辦公室文件《微生物菌種純度檢測技術規(guī)程》（試行）），與保護劑混合均勻，分裝。微生物培養(yǎng)物濃度以細胞或孢子不少于108-1010個/ml為宜（以大腸桿菌為例，為了取得每毫升1010個活細胞菌液2毫升-2.5毫升，只需10毫升瓊脂斜面兩支）。采用較長的毛細滴管，直接滴入安瓿管底部，注意不要濺污上部管壁，每管分裝量約0.1-0.2毫升，若是球形安瓿管，裝量為半個球部。若是液體培養(yǎng)的微生物，應離心去除培養(yǎng)基，然后將培養(yǎng)物與保護劑混勻，再分裝于安瓿管中。分裝安瓿管時間盡量要短，最好在1-2小時內分裝完畢并預凍。分裝時應注意在無菌條件下操作。最后放到凍干機內進行冷凍干燥，即可長期保藏。</p><p><img src="/upload/images/201710/1.jpg"/></p><p><br/></p> </span> </div> <style type="text/css"> .content-text-3 { font-size:16px; color:#333333; line-height:1.8; } .content-text-3:hover { color:#333333; } .content-text-3 img { max-width:100%; } .content-text-3 .content-within-pagination .pagination>li>a, .content-text-3 .content-within-pagination .pagination>li>span { font-size:14px; color:#06276a; border-color:#06276a; border-radius: 0px; background:#ffffff; } .content-text-3 .content-within-pagination .pagination>.active>a, .content-text-3 .content-within-pagination .pagination>.active>a:focus, 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